摘要
目的 建立检测抗中性粒细胞胞浆抗体 (ANCA)的间接酶联免疫吸附试验 (ELISA) ,观察韦格纳氏 /多血管炎、系统性红斑狼疮 (SLE)、肾炎和溃疡性结肠炎患者中ACNA的存在状况。方法 用建立的ELISA法检测 10 0例韦格纳氏 /多血管炎、SLE、肾炎或溃疡性结肠炎患者血清中的ANCA ,同时以特异性MPO ANCA及PR3 ANCA为对照。结果 阳性参考血清批内变异系数 (CV)和批间CV分别为 0 .0 6 1%和 0 .10 2 %。用抗原吸收后吸光度 (A)降低明显。总ANCA在韦格纳氏 /多血管炎患者中的阳性率为 4 3.8% ,高于SLE、肾炎和溃疡性结肠炎患者 (分别为 15 .2 %、2 3.1%和 2 5 % ) ,而 5 2份正常人血清均为阴性。用特异性MPO ANCA及PR3 ANCA与ELISA检测总ANCA结果比较差异无显著性 (P >0 .0 5 )。结论 ANCA可作为韦格纳氏 /多血管炎的血清学标志 ,ELISA检测总ANCA可克服间接免疫荧光法的不足 ,灵敏度高 ,适于大样本的ANCA检测。
Objective To observe the relationship between the anti neutrophilic cytoplasmic antibodies (ANCA) determined with indirect ELISA and the autoimmune disorders, such as Wegener's graulomatosis (WG), systemic lupus erythematosus (SLE), nephritis and ulcerative colitis (UC). Methods Serum samples from 100 patients with WG、SLE、nephritis or UC were measured for ANCA by established method of ELISA. Specific MPO ANCA and PR 3 ANCA were measured simultaneously as controls. Results The intra and inter assay CV s of the reference positive sera were 0.061 and 0.102 respectively. There was a significant decrease in A value after the absorption with antigen. The positive rate of total ANCA in patients with WG was 43.8%(7/16) by ELISA, which was higher than those in patients with SLE (15.2%,7/46), nephritis (23.1%,6/26) and UC (25%, 3/12). The results of ANCA detection in control group (52 cases) were all negative. No significant difference of the positive rate was found among the detecting methods of total ELISA, specific MPO ANCA and PR 3 ANCA( P >0.05). Conclusions ANCA might be used as serologic marker in patients with WG. The method of total antibody ELISA is more sensitive and could overcome the insufficiency of indirect immunofluorescence.
出处
《上海医学检验杂志》
北大核心
2003年第5期285-287,共3页
Shanghai Journal of Medical Laboratory Sciences
