摘要
目的 探讨高草酸尿和输尿管梗阻 (UUO)对鼠肾小管上皮细胞凋亡的影响。 方法 雄性成年Wistar大鼠 4 0只 ,随机分为 4组 ,每组 10只 ,A组为对照组 ,B组为高草酸尿组 ,C组为输尿管梗阻组 ,D组为高草酸尿并输尿管梗阻组。所有大鼠均在术后 2周处死并取左肾 ,常规HE染色检测肾小管间质草酸盐结晶沉着情况 ;原位末端标记法 (TUNEL)检测肾小管上皮细胞凋亡情况。 结果 B组和D组组织切片肾小管腔内可见明显草酸盐晶体沉着 ,A组和C组未见晶体沉着。计数5个高倍镜视野的细胞 ,A、B、C、D各组肾小管上皮细胞凋亡数分别为 1.1± 1.1,31.3± 8.0 ,14 5 .0±33.4 ,2 75 .8± 5 1;A组与各组相比差异均有显著性意义 (P <0 .0 0 1) ,B组与C组、D组相比差异有显著性意义 (P <0 .0 0 1) ,C组与D组相比差异亦有显著性意义 (P <0 .0 1)。 结论 高草酸尿可致肾小管间质草酸盐结晶沉着 ;完全性输尿管梗阻并高草酸尿使肾小管上皮细胞凋亡水平明显增高 ,且造成肾脏损害 ,输尿管梗阻是比高草酸尿更重要的促细胞凋亡因素。
Objective To investigate the role of hyperoxaluria and unilateral complete ureteral obstruction in the development of renal tubular epithelial cell apoptosis. Methods Forty male Wistar rats were divided into four groups randomly,with ten rats in each.The groups were named A(control),B(hyperoxaluric),C(obstructive) and D(hyperoxaluric+obstructive).The rats were sacrificed 2 weeks after operation;left nephrectomy was then performed.The tissue sections obtained from kidneys were examined under light microscopy for the presence and the degree of crystal deposition in the tubular lumen.Apoptotic changes in renal tubular cells were examined using the terminal deoxynucleotidyl transferase(TdT)-mediated dUTP in situ nick and labeling (TUNEL) method. Results Oxalate crystal deposition was evident in the renal tubular lumen in groups B and D,while there was no evidence in groups A and C.With respect to apoptotic changes,the mean values of the apoptotic cell count were 1.1±1.1 in A and 31.3±8.0 in B.The values for C and D were 145.0±33.4 and 275.8±51,respectively.There was statistically significant difference between all goups ( P <0.001) except for that between C and D ( P <0.01). Conclusions Oxalate crystal deposition in the tubular lumen results from hyperoxaluria;hyperoxaluria with complete ureteral obstruction induces an increased level of apoptosis,which is responsible for renal damage.The study suggests that ureteral obstruction is a more important factor for apoptosis than hyperoxaluria.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2003年第9期608-610,共3页
Chinese Journal of Urology
