摘要
目的 了解低氧诱导因子 1α(hypoxiainduciblefactor- 1α ,HIF - 1α)在实验性脊髓损伤中的表达规律并探讨其在脊髓损伤中的作用。 方法 通过逆转录聚合酶链式反应 (RT -PR)、原位杂交和免疫组化方法 ,分别从mRNA和蛋白水平研究HIF - 1α在脊髓损伤后不同时段的表达情况。结果 HIF - 1α的mRNA和蛋白在损伤脊髓中的各种细胞类型中广泛表达 ,而且表达较正常脊髓明显增高 (P <0 .0 5 ) ,其中蛋白表达高峰 (伤后 1d)较mRNA表达高峰 (伤后 3d)提前。 结论 脊髓损伤中存在缺血缺氧损害 ,缺氧环境可通过诱导转录和增强蛋白稳定性来增加HIF - 1α蛋白量 ,这可能与HIF - 1α的下游基因激活和脊髓组织细胞耐受缺氧环境有关。
Objective To explore the expression pattern and effects of hypoxia inducible factor-1α (HIF-1α) in experimental spinal cord injury. Methods The expression of HIF-1α at various time was detected at levels of mRNA and protein by using methods of reverse transcription-polymerase chain reaction (RT-PCR),in situ hybridization (ISH) and immunohistochemistry. Results HIF-1α expressed more significantly at levels of mRNA and protein in all kinds of cells in the injured spinal cord than in the normal spinal cord (P<0.05). The protein expression of HIF-1α reached climax at the first day after operation, earlier than the mRNA expression (at the third day after operation). Conclusions Hypoxia-ischemia damage exists in spinal cord injury. Hypoxia circumstance can increase protein content by inducing transcription and reinforcing stability of HIF-1α protein, as may relate to the activation of HIF-1α downstream genes and the cell tolerance of spinal cord tissue to hypoxia circumstance.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2003年第9期544-547,共4页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目 ( 3 9970 75 5 )
