摘要
目的 :了解局灶硬化性肾小球肾炎 (FGS)模型小鼠的细胞因子表达谱 ,以及复方积雪草对其细胞因子网络的表达调控作用。方法 :通过BALB c小鼠尾静脉一次性注射阿霉素 (11mg·kg-1) ,诱导成FGS肾炎模型 ,以复方积雪草制剂灌胃治疗 4wk ,分别取 1、4wk肾组织 ,提取其总RNA ,通过反转录掺入荧光素CY3 dUTP CY5 dUTP ,转录产物与细胞因子基因芯片杂交 ,芯片扫描并进行图像分析。结果 :基因表达谱芯片检测表明 :复方积雪草可抑制PDGF α、PDGF β、PDGF受体、VEGFmRNA的表达 ;能抑制IL 9、IL 7R2、MIP等因子的mRNA的表达 ;经复方积雪草刺激后 ,肾组织中癌基因c Myc ,Jun表达被抑制。结论 :复方积雪草具有抗肾纤维化作用 。
AIM: To study the cytokine gene expression profile in mice m od el with focal glomerulosclerosis (FGS) nephritis and the mechanism of Ce ntella asiatica compound on cytokines network. METHODS: FGS model mice of BALB/c were induced by adromycin (ADR, 11 mg·kg -1 ), and then treated with Centella asiatica compound for four weeks . The values of total RNA were prepared from the kidney tissue at the end of fir st week and fourth week. The cDNA retained by the reversal react and labeled wit h CY3-dUTP/ CY5-dUTP in fluorescence, hybridized with the cytokines genes micr oarray. The chips were scanned in SCANARRAY 3000 and the images were analyzed by Image 4.0 software. RESULTS: The expression of PDGF-α, PDGF- β, PDGF-R and VEGF mRNA were down-regulated after treated by Centella asiatica compound;the expressions of IL-9, IL-7R2, and MIP were down -regulated after treated by the compound. At the same time, through the stimula tion of the compound, the expressions of c-Myc and Jun in kidney were restraine d. CONCLUSION: Centella asiatica compound has a function of anti-glomerulosclerosis, and it may be a result of various cytokines worked together.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第6期638-641,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
杭州市科委重大项目资金项目 (№ 2 0 0 1113A0 9)
