摘要
以扇叶病株为材料,根据病毒编码外壳蛋白的核苷酸序列,设计特异引物P_1(1,064—1,083),P_2(762—781),建立了以RNA为模板的GFLV的RT-PCR检测体系。PCR产物电泳谱带明显,与预期的引物应扩增321 bp片段完全吻合。并利用建立起来的技术体系,对田间葡萄进行了随机取样检测,取得了很好效果。此方法操作简单,检测周期短,准确率高,具有广泛和更进一步的应用价值。
RT-PCR primers were designed based on the GFLV coat protein region, Located at the 3' end of RNA2. Primer 1 is complementary to nucletides 1,064-1,083,' Primer 2 corresponds to nu-cleotides 762-781. Preliminary RT-PCR experiment with RNA showed that these primers are suffi cient to amply DNA of the expected size(321 bp). In virtue of easy-manufacture,short-period,high-accuracy , think of the potential value on further application, RT-PCR diagnosis has important value of theory and practice. It will give great impetus on virus-free manufacture.
出处
《西北农业学报》
CAS
CSCD
2003年第3期81-85,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
农业部"九五"课题(编号:垦-06-24)
兵团科委资助项目(NKB02SDXNK01SW)
