摘要
应用合理的免疫程序,用猪瘟抗原免疫蛋鸡,并以水稀释法、聚乙二醇萃取法结合透析对所产蛋的卵黄部分进行处理,成功地提取到抗猪瘟IgY(SF-IgY),获得率为6.83mg/mL。该制剂纯度为45.14％,能中和1000MID猪瘟兔化种毒,用于紧急治疗猪瘟后,临床应用效果确实。
Layer hens were immunized with swine fever antigen under proper immunity schedule. The egg yolk was collected and tested after the treatments with water dilution, PGE extraction and dialysis. Then the IgY preparation against swine fever (SF-IgY)was successfully produced, with the purification degree of 45. 14% and production rate of 6. 83 mg/mL. The SF-IgY product could neutralize 1000MID Swine Fever virus hatched with rabbit and showed reliable effect in urgent clinical treatment against Swine Fever.
出处
《西北农业学报》
CAS
CSCD
2003年第3期36-38,共3页
Acta Agriculturae Boreali-occidentalis Sinica
基金
杨凌职业技术学院科研基金(B52002002)
