摘要
我们首次采用标记和定位抗脑抗体(ABA)抗血清,观察其在听源性癫痫大鼠(GEPRs)不同脑区和神经结构中的分布,结合组织病理学观察,间接定位与导致痫性放电有关的及附着有ABA的特殊脑抗原结构部位(即癫痫抗原,EPI-AG),直接从免疫学角度揭示癲痫(EPI)发病机制.结果显示,抽搐组GEPRs的额顶叶深层皮质、海马回、齿状回、丘脑、小脑颗粒细胞层、丘脑之室管膜及海马之软脑膜抽搐或抽搐预激敏感脑区呈ABA阳性反应.其中又以纤维型星形胶质细胞突及其分枝末端最明显.组织病理学结果也显示ABA阳性脑区中有活跃的纤维型星形胶质细胞.因此,纤维型星形胶质细胞成份,不仅参与EPI免疫发病的主要过程,还可能做为EPI-AG引发EPI免疫反应,从而导致痫性放电甚或临床发作.
Using the method of labelling and locating the distribution of antibrain antibodies (ABA) in different cerebral tissue sections of genetically epilepsyprone rats combined with histopathological observations,we for the time indirectly ocated the antigens related to epileptic discharges, in our exploration of the pathogenesis of the EPI from an immunological point of view. The result showed that ABA reaction was mainly limited to the specific regions of the hippocampus, dentat gyrus,thalamus, the deep layer of the frontal and portal coticus.The mostarked reaction occurred in fibrial astocytes,which showed an obvious hyperexcitable status and a closer relationship to the combination of neurons and capillaries. This result suggests that the primary GEPRs possible have the mechanism of easily kindling epileptic dischargesand that the key strusture was located in the above ABA positive cerebral regions.The fibrial astrocyte portions not only take part
in the immune process but possibly act as epileptic antigen to irritate the immune reaction and finally cause epileptic discharges of neurons or clinical seisures.
出处
《青岛医学院学报》
1992年第2期93-97,共5页
Acta Academiae Medicinae Qingdao Universitatis
关键词
癫痫
免疫技术
抗原
脑
动物
epilepsy
histochemistry
immunological
antigen
cerebral
laboratory
