摘要
目的 研究增殖性疤痕中血管内皮细胞生长因子(VEGF)的表达,及其与疤痕表皮细胞增殖和血管新生的关系。方法 应用免疫组化和RT-PCR法,以正常皮肤、正常疤痕为对照,检测增殖性疤痕中VEGF表达,并研究VEGF与表皮细胞增殖、血管新生的相关关系。结果 增殖性疤痕内VEGF蛋白及VEGFmRNA表达明显升高,与正常疤痕、正常皮肤有显著差别。VEGF主要由疤痕表皮角朊细胞分泌,且VEGF蛋白和VEGFmRNA的表达与疤痕组织表皮细胞增殖、血管新生高度相关。结论 增殖性疤痕中VEGF蛋白和VECFmRNA的表达与疤痕增殖密切相关。
Objective To study the expression of vascular endothelial growth factor (VEGF)in proliferative scars, and relationship between the keratinocytes in epidermis and revascularization in scar der-mis . Methods VEGF protein secretion and VEGFmRNA expression were detected by immunohisto-chemistry and RT - PCR,and were compared with cell proliferation and revascularization. Results It was found that the VEGF expressions in hypertrophic scar and keloid had the same determinations , VEGF protein and VEGFmRNA were highly increased . VEGF were almost secreted by keratinocytes in the epidermis. On the contrary, in the normal scar and normal skin, the VEGF expressions were at extremely low levels. According to the correlation test, the VEGF expression showed significant correlation with cell proliferation in keratinocytes and microvascular density. Conclusion Highly expression of VEGF in proliferative scars may play a role in the formation of keloid and hypertrophic scar.
出处
《上海第二医科大学学报》
CSCD
2003年第5期399-402,429,共5页
Acta Universitatis Medicinalis Secondae Shanghai
