摘要
目的 探讨锌对γ射线诱导的体外培养小鼠骨髓细胞辐射损伤的影响及可能的机制。方法 分离小鼠骨髓细胞并悬浮于RPMI 16 4 0培养基中 ,分别将锌按 2 0 ,2 0 0 ,4 0 0 ,80 0 μmol/L终浓度加入细胞培养液中 ,5Gy60 Coγ射线照射骨髓细胞后置于 37℃、5 %CO2 细胞培养箱中培养 ,16h后终止培养 ,分别测定有核细胞数、细胞DNA含量 ,并用流式细胞术和免疫组织化学技术测定细胞周期、细胞凋亡和基因表达的改变。结果 5Gy60 Coγ射线照射体外培养的小鼠骨髓细胞后 ,骨髓有核细胞数、DNA含量均明显下降 ;细胞凋亡率增加 ,细胞发生S期阻滞 :bcl- 2蛋白表达下降 ,而 p2 1蛋白的表达升高。照前在培养液中加入一定浓度的锌后 ,凋亡率下降 ,DNA含量升高 ,有核细胞数增加 ,细胞bcl- 2蛋白表达升高而p2 1表达下降 ,细胞周期阻滞得到缓解。结论 锌可能通过影响细胞凋亡、细胞周期和基因表达而发挥辐射损伤防护作用。
Objective To study the effects and possible mechanisms of zinc on the γ-ray induced irradiation damage of the bone marrow(BM) hematopoietic cells in mice.Methods The BM cells(BMC) were separated from mice and were suspended in RPMI 1640 mediums.Zinc was added to the mediums,whose final concentrations in the mediums were 20,200,400,800?μmol/L respectively.Then the cells were irradiated by 60 Co γ-ray,the dose was 5Gy.16 hours later,the number of BM karyote and the contents of DNA of BMC were measured.By flow cytometry(FCM) and immunihistochemistry technique,the rates of cell apopotosis,the changes of the cell cycle and the expression of genes were examined respectively.Results After irradiation,the number of BM karyote and the contents of DNA were decreased;the rates of cell apoptosis were increased.BM cells were blocked in S phase,lower expression of bcl-2 protein and higher expression of p21 protein were observed in BM cells.When Zn 2+ were added to the cell cultures before irradiating,the number of BM karyote and the contens of DNA were increased;the rates of cell apoptosis were decreased.The block of the cell cycle was improved significantly,the expression of bcl-2 protein was increased and the expression of p21 protein was decreased.Conclusion Zinc may protect mice BM cells against radiation damage in vitro by its effects on cell apoptosis,cell cycle and expression of genes.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第9期1081-1083,共3页
Chinese Journal of Public Health
关键词
锌
Γ射线
骨髓细胞
凋亡
细胞周期
基因表达
zinc
γ-ray
bone marrow cell
apoptosis
cell cycle
gene expression
