摘要
目的 探讨错配修复基MLH1和微卫星BAT2 6不稳定性在原发性肝癌发生中的作用。方法 采用二维电泳和DNA测序方法检测原发性肝癌错配修复基hMLH1突变 ;采用PCR方法检测细胞核BAT2 6微卫星位点不稳定性。结果 5 2例肝癌未发现有hMLH1突变者 ,有 4例BAT2 6位点检出nMSI,阳性率为 7.7%。结论 原发性肝癌的发生并非通过微卫星不稳途径。
Objective To evaluate the role of microsatellite instability at BAT26 and mismatch repairing gene hMLH1 mutation in the occurrenc and development of hepatocellular carcinoma. Methods MSI at BAT26 was detected by PCR SSCP; The hMLH1 mutation was detected by two dementional electrophoresis and DNA sequencing methods. Results Fifty two cases of hepatocellular carcinoma were studied for hMLH1 mutation and MSI at BAT26. The MSI at BAT26 was detected in only 4 of 52 (7.7%) hepatocellular carcinoma cases and no hMLH1 mutation was observed in these cases. Conclusions The development of hepatocellular carcinomas is independent on the MSI pathway.
出处
《重庆医学》
CAS
CSCD
2003年第9期1170-1171,共2页
Chongqing medicine
关键词
肝癌
微卫星不稳
hMLHl突变
hepatocellular carcinoma
microsatellite instability
hMLH1 mutation
