摘要
目的 观察腹腔感染时肺组织中肿瘤坏死因子 -α(TNF -α)和白介素 -8(IL -8)的变化 ,探讨其作用机制。方法 采用大鼠盲肠结扎并穿孔造成腹腔感染。分别在 0、2 4、48、72、96、12 0h处死一组大鼠 ,检测肺毛细血管通透性 ,取支气管肺泡灌洗液(BALF)进行细胞学分析 ,检测血浆、肺组织和BALF的TNF -α和IL -8的含量。结果 肺毛细血管通透性和BALF的中性粒细胞百分率逐渐增加 ,时间越长越明显。血浆、肺组织和BALF的TNF -α、IL -8逐渐增加 ,肺组织和BALF的TNF -α、IL -8分别显著相关 ,两者分别与血浆的TNF -α、IL -8无明显相关性。结论 TNF -α和IL -8在腹腔感染的早期阶段就参与了肺的炎症反应 ,检测BALF的TNF -α和IL
Objective To explore the effective mechanism of tumor necrosis factor-α(TNF-α) and interlukin-8(IL-8) in experimental lung damage by observing changes of TNF-α and IL-8 concentration in lung tissue during abdominal infection. Methods The cecal ligation and perforation were utilized to make the abdominal infection in rats.The rats were sacrificed respectively at 0,24,48,72,96 and 120 hours after operation. The pulmonary vascular permeability and the amount of different cells in bronchoalveolar lavage fluid(BALF) were examined. The concentrations of TNF-α and IL-8 in BALF, lung tissue and plasma were assayed. Results The pulmonary vascular permeability and the neutrophil percentage of BALF increased progressively. The concentrations of TNF-α and IL-8 significantly increased in plasma, lung tissue and BALF. Significant correlation was respectively present between the TNF-α levels and the IL-8 levels in lung and BALF. The levels of TNF-α and IL-8 in lung and BALF were not correlated respectively with those in plasma. Conclusions TNF-α and IL-8 may be implicated in the inflammatory reaction of lung during early stage of abdominal infection. Lung injury derived from intestinal infection could be early discovered through assaying TNF-α and IL-8 concentration in BALF.
出处
《中国医师杂志》
CAS
2003年第10期1334-1336,共3页
Journal of Chinese Physician
