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枯草芽孢杆菌转酮醇酶变异株C1-B941菌种特性的初步研究

Study on Characteristics of the Transkelolase Mutant Bacillus subtilis
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摘要 C1-B941是一株枯草芽孢杆菌(Bacillus subtilis)的转酮醇酶(Transketolase,TKT)变异株,与亲株C1相比,其在菌体形态及代谢特性上发生了显著变化,如对戊糖不利用,对葡萄糖等利用变弱,碳源利用存在抑制,表现为芳香氨基酸合成途径缺陷,对数期细胞聚集成链,孢子形成能力下降,在发酵培养基中可以积累D-核糖,发酵液具有广泛的用途。 C1-B941 is a transketolase mutant of Bacillus subtilis., which is differentiated remarkably with the parent strain C1in the characteristics of cell morphlogy and metabolism. The results showed that: not utilizing pentoses; or weakening thecarbohydrates such as D-glucose utilization or inhibition interaction on carbohydrates ; source there would be defects in synthesisof aromatic amino acids, chains formation during exponential cell growth; and lowering of sporulation capability; It was possibleto accumulate D-ribose in the ferment medium, so as to make the fermented liguid useful in a lorge scale.
出处 《食品科学》 EI CAS CSCD 北大核心 2003年第9期44-48,共5页 Food Science
关键词 枯草芽孢杆菌 转酮醇酶变异菌株 C1—B941 菌种特性 D-核糖产生菌 D-ribose-producing strain Bacillus subtilis transketolase mutant propertise
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参考文献6

  • 1De wulf P,& Vandamme E J.Production of D-ribose by fermentation[J].Appl Micro Biotechnol,1997,48:141-148.
  • 2Sasajima K et al.Method for the production of D-ribose[P].1976, US patent 3970522.
  • 3Sasajima K ct al.Carbohydratc metabolism mutants of a Bacillus species.Part I:Isolalion of mutants and their inosinc formation[J].Agri Biol Chem,1970,34:381-389.
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