摘要
从腹泻仔猪粪样中分离出一株猪轮状病毒(Na 86毒株),经在MA104细胞上连续传代后,对其不同代次的细胞毒进行了核酸图谱的分析、毒力及抗原性测定。结果表明,随着培养代次的增高,病毒核酸图谱有所变化,同时对未吮初乳仔猪的致病力逐渐下降,传至82和90代的细胞毒已完全失去了致病性,它既不引起仔猪腹泻也未使肠道组织发生病理变化,在未吮初乳猪肠道内连传三代,未见毒力反强;通过血凝特性的测定及高免血清的交互琼脂扩散试验等证明90代病毒,仍具有原来的良好抗原性。
A strain of porcine rotavirus isolated from the feces of diarrheal piglets in Jiangsu province was replicated serially in MA104 cell cultures.The genome, pathogenicity and antigenicity of the cell-cultures of different passages were Studied. Same variation in the PAGE pattern of genomic RNA from the advanced passages of cell cultures were noticed. The pathogenicity of the virus became weaker gradually, the cell culture of the 90 th passage almost lost its pathogenicity entirely assh-own by the fact that neither diarrhea nor pathologic changes were induced in the newborn piglets fed with the culture, and it remained safe or nonpathogenic even it was passed through the alimentary canal of newborn piglets three times successively. The 90th passage virus remained its antigenicity as shown in the detection of the viral hemagglutination test and cross reaction with hyperimmune serum in agar diffusion test.
出处
《江苏农业学报》
CSCD
1989年第4期46-53,共8页
Jiangsu Journal of Agricultural Sciences
基金
本课题为国家自然科学基金资助项目
关键词
猪
轮状病毒
弱毒株
培育
Rotavirus
Genomic RNA
Pathogenicity
Antigenicity
Pathologic change
