摘要
用组织培养法筛选出的抗白叶枯病菌及对照的愈伤组织再生植株,分别种植122及53株。其中发生表型变异的依次为115及27株,各占其总株数的94.2％和51.9％。变异频率显著增加,变异表型多样。再生植株自交后代R_1-R_5代的性状,一般是稳定可遗传的。有一个株系的R_2代发生分离。
Calli from dehulled seeds of a rice variety Nangeng 34 (Oryza sativa subsp. Keng) susceptible to bacterial leaf blight (Xanthomonas oryzae) were co-cultured with Xanthomonas oryzae pathogen in order to establish cell clones and regenerate plants resistant to the disease. The same kind of calli was also cultured in the absence of the pathogen as a control. As a result, 122 and 53 regenerated plants were obtained from the co-cultured calli and the control respectively, which had 94.2% and 51.9% of their respectiv totals showing various phenotypical variations, including disease resistance, polyploidy, sterility, more seeds per panicle, lower or higher seed weight, shorter plants and panicles, shorter and narrower flag leaves, longer growth duration, compacter plant type, straight and erect leaves. This indicates that variation frequencies in the regenerated plants were significantly increased by co-culture of the calli with the pathogen and its secretion. The performances of the selfed progenies (R1-R5) of the regenerated plants were observed to be genetically stable, except one of the plant lines which segregated.
出处
《江苏农业学报》
CSCD
1989年第1期24-30,共7页
Jiangsu Journal of Agricultural Sciences
关键词
组织培养
水稻
再生植株
白叶枯病
Rice
Tissue culture
Regenerated plants
Bacterial leaf blight
Genetic variation
