摘要
①目的 了解肝康饮对实验大鼠肝纤维化的作用 ,并探讨其可能的作用机制。②方法 应用四氯化碳诱导建立大鼠实验性肝纤维化模型 ,肝康饮组以肝康饮治疗 ,检测治疗后大鼠血清总蛋白 (TP)、清蛋白 (ALB)、碱性磷酸酶 (AKP)、丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)水平以及血清层粘连蛋白 (LN)、透明质酸 (HA)、前胶原Ⅲ型肽 (PcⅢ )含量 ,光镜下行肝脏组织形态学观察 ,并与正常组和模型对照组进行比较。③结果 肝康饮组血清TP、ALT、AST、AKP水平及HA、LN含量均较模型对照组降低 ,差异有显著性 (F =5 .5 3～ 90 .4 5 ,q =5 .88～ 17.6 2 ,P <0 .0 1) ;血清ALB水平及PcⅢ含量均较模型对照组升高 ,差异有显著性 (F =19.5 0、16 .2 9,q=5 .80、7.2 0 ,P <0 .0 1)。④结论 肝康饮能减轻实验大鼠肝细胞变性、坏死及纤维化程度 。
Objective To study the anti-fibrosis effect of GAN-KANG-YIN, a herbal decoction, on the liver of experimental rats and its possible mechanism. Methods The hepatic fibrosis of rat models were established by using carbon tetrachloride. The rats were then treated with the decoction. After the treatment, the levels of serum total protein(TP), alkaline phosphatase(AKP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), serum laminin (LN), hyaluronic acid (HA) and procollagen peptide type Ⅲ (Pc Ⅲ) were measured. The liver tissue was observed microscopically. The results were compared with normal group and model-control group. Results GAN-KANG-YIN markedly reduced the levels of TP, ALT , AST, AKP, LN and HA in experimental group as compared with model-control group, the difference was significant (F=5.53-90.45, q= 5.88-17.62, P<0.01) . The levels of ALB and Pc Ⅲ were higher than model-control group, the difference was significant ( F= 19.50, 16.29, q=5.80,7.20, P<0.01). Conclusion GAN-KANG-YIN may relieve the degeneration, necrosis and fibrosis of liver cells of experimental rats. The decoction has an effect of prevention and treatment for hepatic fibrosis caused by carbon tetrachloride.
出处
《齐鲁医学杂志》
2003年第3期251-253,255,共4页
Medical Journal of Qilu
