摘要
①目的 探讨 p16基因 5′CpG岛高甲基化在妇科恶性肿瘤发生发展中的作用。 ②方法 采用以PCR为基础的甲基化分析法 ,检测p16基因第 1外显子 5′CpG岛在 34例子宫颈癌、4 0例子宫内膜癌和 4 0例上皮性卵巢癌组织及其相应正常组织中的高甲基化情况。③结果 正常组织中均未见 p16基因第 1外显子 5′CpG岛的高甲基化 ,子宫颈癌、子宫内膜癌和上皮性卵巢癌组织中的高甲基化率分别为 2 9.4 %、30 .0 %和 10 .0 %。子宫颈癌、子宫内膜癌的高甲基化率高于正常宫颈组织 (χ2 =5 .4 0、4 .33,P <0 .0 5 )。子宫颈癌和子宫内膜癌组织的高甲基化与临床分期和组织学分级有关 (χ2 =4 .2 9～ 9.78,P <0 .0 5 )。④结论 p16基因
Objective To study the role of hypermethylation of 5′CpG island in p16 gene in the occurrence and development of gynecologic malignancies. Methods PCR-based methylation analysis was used to detect the hypermethylation status of 5′CpG island of exon 1 in p16 gene in 34 cervical, 40 endometrial and 40 ovarian carcinomas and their corresponding normal tissues. Results No hypermethylation of 5′CpG island of p16 gene was detected in normal cervical, endometrial and ovarian tissues. Hypermethylation rate in cervical , endometrial ,and ovarian carcinomas was 29.4%, 30.0% and 10.0%, respectively. Hypermethylation in cervical and endometrial carcinomas was significantly higher than their normal tissues (χ 2=5.40, 4.33, P<0.05).In cervical and endometrial carcinomas, hypermethylation was significantly associated with clinical staging and histological grading (χ 2= 4.29-9.78, P<0.05). Conclusion Hypermethylation of p16 gene plays an important role in the occurrence and progression of cervical and endometrial carcinomas.
出处
《齐鲁医学杂志》
2003年第3期227-229,共3页
Medical Journal of Qilu
