摘要
①目的 探讨肺癌组织中血管内皮生长因子受体KDR的表达与临床病理因素的关系。②方法应用免疫组织化学PicTureTM PV90 0 0法 ,测定 75例肺癌标本中血管内皮生长因子受体KDR的表达。③结果KDR在肿瘤细胞中的阳性表达率高于间质纤维母细胞 ,差异有显著性 (χ2 =5 .88,P <0 .0 5 )。肿瘤细胞及纤维母细胞中KDR的阳性表达率在不同年龄、不同性别及不同病理类型、不同病理分级之间差异均无显著性 (χ2 =0 .6 6 8～3.2 34,P >0 .0 5 ;P =0 .786 )。KDR在不同大小的肿瘤组间肿瘤细胞中的表达差别有显著性 (χ2 =7.2 88,P <0 .0 5 ) ,而在纤维母细胞中的差别无显著性 (χ2 =2 .5 5 8,P >0 .0 5 )。淋巴结有转移组的肿瘤细胞及纤维母细胞中KDR的阳性表达率均高于无转移组 ,差异有显著性 (χ2 =9.316、8.833,P <0 .0 5 )。不同术后生存期组中 ,KDR的表达在肿瘤细胞及纤维母细胞中均有极显著差别 (χ2 =16 .19、19.19,P <0 .0 0 1)。④结论 利用免疫组织化学等方法检测肺癌组织中KDR的表达 ,可为肺癌的预后评估及治疗提供较可靠的指标。
Objective\ To investigate the expression of vascular endothelial growth factor receptor 2(VEGFR 2) KDR in lung cancer and its relationship with clinical pathology.\ Methods\ The expression of KDR was detected by immunohistochemical PicTure TM PV9000 method in 75 patients with the disease. \ Results\ The positive rate of KDR was significantly higher in tumor cells (TC) than in fibroblasts (FB) ( χ 2=5.88,P <0.05). In both TC and FB, no significant difference was found between them in the expression of KDR in terms of age, sex, pathological typing or histological grading( χ 2=0.668-3.234,P >0.05; P =0.786).In TC, it was obviously that the positive rate of KDR was different in different sizes of tumor( χ 2=7.288,P <0.05),but was not in FB( χ 2=2.558,P >0.05). The positive rate of KDR in both TC and FB was significantly higher in cancer with lymph node metastasis than that of without( χ 2=9.316,8.833, P <0.05). The positive rate of KDR in TC and FB was significantly different among different postoperative survival period of time( χ 2=16.19,19.19, P <0.001).\ Conclusion\ The detection of the expression of KDR in tissue of lung cancer by immunochemical technique may provide a reliable parameter, which opens a new way for evaluation of the prognosis and development of pulmonary carcinoma.
出处
《青岛大学医学院学报》
CAS
2003年第3期307-309,共3页
Acta Academiae Medicinae Qingdao Universitatis
