摘要
本研究的目的是探讨唯酶抗体的血型血清学特性及在临床输血中的意义 ,为安全输血提供依据。采用含唯酶抗体的病人血清在多种介质中与ABO同型献血者红细胞、谱细胞和自身细胞进行反应 ,并通过吸收释放试验来判断唯酶抗体的血型血清学特性。结果表明 ,该唯酶抗体仅在木瓜蛋白酶介质中与献血者红细胞和谱细胞反应呈阳性结果 ,且自身对照亦呈阳性反应 ,而在其它介质中均呈阴性反应。吸收实验后抗体效价降低 ,采用乙醚放散 ,放散液中未测出抗体。该病人输注ABO及Rh同型献血者红细胞 60 0ml后无任何不良反应。结论 :该例唯酶抗体仅在木瓜蛋白酶介质体外检测中表现出凝集 。
The objective of this study was to investigate the serologic characters of enzy me-only red cell antibody and its clin ical significance, and to provide basis for the safety of blood transfusion. Th e patient serum containing enzyme-only antibody was used to react with the red cells of donors, panel cells and auto-cells in various medium. Absorption an d elution test were also per formed. The results showed that this blood sample was found to cont ain an antibody that reacted with donor red cells and panel cells only in papain medium, but was not demonstrable by indirect antiglobulin test and other method s. Decl ine of antibody titers was observed af ter absorption test, but antibody activity was not detected in the elute. The pa tient underwent transfusion with 600 ml of Rh type identical RBCs, without any hemolytic transfusion reaction. In conclusion, enzyme-only antibody usually doe s not lead to hemolytic transfusion reaction.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第5期527-529,共3页
Journal of Experimental Hematology
关键词
唯酶红细胞抗体
血型血清学
木瓜蛋白酶
溶血性输血反应
enzyme-only red cell antibody
blood group serolog y
papain
hemolytic transfusion reaction
