摘要
本文通过一系列试验指出:1、硫钼酸盐可明显地减少大鼠的肝、肾、肠内与MT结合的铜浓度,尤以肝、肾最明显,而对Zn-MT的结合作用没有明显影响。硫钼酸盐对牛的肝细胞浆液中与MT结合的铜有类似的影响。同时它还有延迟静脉注射的铜进入肝浆与MT结合的作用。2、饲料中添加钼和硫后,于第1个月内,肝内MT浓度在肝铜大量丢失之初,由于肝内可动员铜大量释放,Cu-MT浓度增加,随后则逐渐降低。3、铜可以诱导缺铜牛肝浆内MT的合成,这种合成作用在静脉注射铜后6-10h开始,于24～48h达到峰值浓度。铜诱导了肝浆液中MT合成以后,不仅Cu-MT浓度增加,而且Zn-MT浓度也增加。因而提示MT上可能存在着至少两个结合部位,一个结合铜,另一个结合锌。
Several experiments have been done, the results here are shown that: Copper combined to metallothionein in the cytosol of liver, kidney and intestine of anesthetic rats can bedecreased by trithiomolybdate administered from deudenum especially in liver and kidney. However, no effects on the combination of zinc to metallotionein. Similar results were obtained on the copper combined to metallotbionein in the cytosol of cattle liver. Molybdate may have a function to delay the copper goes to liver from plasma to combined to metallothionein or induce the metallothionein' synthesis. Copper concentration combined to metallothionein in the cytosol of liver of cattle fed a ration supplemented with molybdate and sulphur would increase in the first month of experiment, because of the increasing of labile copper in the liver. Then, Cu-MT. concentration would decrease gradually after the first month till the end of experiment. Metallothionein can be induced to synthesize by copper injection intravenously. The synthesis occured 6 hours after copper injection. A peak value is at 24-48 hours. After metallothionein synthesized, both the concentration of Cu-MT and Zn-MT increased. It seems that there are twocombining sites on the moleculeof metallothionein, one is for copper, anothor is for zinc.
出处
《江苏农学院学报》
CSCD
1989年第1期41-46,共6页
Jiangsu Agricultural Research
关键词
咪噻宁
大鼠
娟姗牛
肝浆液
铜
copper
molybdenum
thiomolybdate
metanothionein rat jersey cytosol liver
