摘要
采用实时荧光定量PCR技术 ,通过使用特异的引物和探针 ,对大豆中的内源基因Lectin和转基因大豆中的外源基因EPSPS进行了定量检测 ,建立了Monsanto公司生产的商业化转基因大豆Roundup Ready○R的定量PCR检测方法。该方法的检测灵敏度 <0 0 1% ,是国际上设定的转基因最低限量的 10
A quantitative method was developed to detect transgenic 'Roundup Ready' soybean (Monsanto) by real time quantitative PCR Special primers and probes were used to amplify the endogenous gene lectin and exogenous gene CP4EPSPS The detection limit of this method was 0 01% which was 100 times lower than the lowest international labeling threshold The statistical analysis showed that the accuracy and precision of this method was in agreement with other quantitative GMO detection systems
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2003年第8期65-69,共5页
Food and Fermentation Industries
基金
科技部社会公益研究专项资金项目
