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Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection

Determination of Amino Acids in an Individual Erythrocyteby Capillary Electrophoresis with Intracellular FITC-derivatization and Laser-induced Fluorescence Detection
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摘要 A novel approach for analysis of amino acids in individual erythrocytes was established. In this method, the derivatization reagent was introduced into the living cells by electroporation. After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection. A novel approach for analysis of amino acids in individual erythrocytes was established. In this method, the derivatization reagent was introduced into the living cells by electroporation. After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection.
出处 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第9期952-954,共3页 中国化学快报(英文版)
基金 supported by the National Natural Science Foundation of China,the State Key Laboratory of Microbial Technology,Shandong University.
关键词 Capillary electrophoresis laser-induced fluorescence detection single cell analysis amino acid. Capillary electrophoresis, laser-induced fluorescence detection, single cell analysis, amino acid.
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参考文献3

  • 1S. D. Gilman, A. G. Ewing, Anal. Chem., 1995, 67, 58.
  • 2Q. Dong, X. Wang, L. Zhu, W. Jin, J. Chromatogr. A, 2002, 959, 269.
  • 3Q. Dong, W. Jin, Electrophoresis, 2001, 22, 2786.

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