摘要
目的 :分析长期服用锂盐对中枢神经系统ERK 1/ 2信号传导通道活性以及Bcl 2家族蛋白表达的影响 ,探讨锂盐治疗躁狂抑郁症作用的可能分子机制。方法 :将 40只健康的雄性Wistar大鼠随机均分为锂盐组和对照组。锂盐组大鼠用每公斤含 2 .4g碳酸锂的饲料喂养 ,对照组大鼠用常规饲料喂养 ,4周后取大鼠海马和额叶制备蛋白样本 ,WesternBlotting分析海马和额叶组织MEK ,ERK 1/ 2 ,RSK1,CREB的磷酸化水平 (活化程度 )以及Bcl 2和Bax蛋白的表达水平。结果 :与对照组比较 ,锂盐增强大鼠海马和额叶MEK ,ERK 1/ 2 ,RSK1和CREB的活性 ,上调海马和额叶Bcl 2表达 ,下调海马和额叶Bax表达。结论 :长期服用锂盐激活中枢神经系统ERK 1/ 2信号传导通道 ,调节中枢神经系统Bcl 2家族蛋白表达 ,这些作用可能与锂盐抗躁狂抑郁症的作用有关。
Objective To investigate the effects of chronic in vivo lithium administration on ERK-1/2 signal pathway and the expression of Bcl-2 family proteins, and to elucidate the molecular mechanisms underlying the therapeutic effects of lithium on the manic depression. Methods Forty male Wistar rats were evenly divided into 2 groups. One group was treated with 2.4 g/kg of Li 2CO 3 for 4 weeks. The other group were given with normal chow. By the end of the 4th week, rat brains were removed immediately on decapitation and dissected on ice. The total proteins of rat brain hippocampus and frontal cortex were prepared. Levels of the prylated, active forms of MEK, ERK-1/2, RSK1 and CREB, and the expression levels of Bcl-2 family were assayed by Western Blotting. Results Lithium increased the activities of MEK, ERK-1/2, RSK1,and CREB,up-regulated the expression of Bcl-2, and down-regulated the expression of Bax in rat brain hippocampus and frontal cortex. Conclusion Chronic in vivo lithium administration activates ERK-1/2 signal pathway and regulates the expression of Bcl-2 family proteins in the central nervous system, which may associate with the therapeutic effects of lithium in the treatment of manic depression.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第4期330-334,共5页
Bulletin of Hunan Medical University
基金
教育部跨世纪优秀人才培养计划基金 (教技函 [2 0 0 2 ] 48)
教育部留学回国人员科研启动基金项目 (教外司留 [2 0 0 2 2 47] )
湖南省卫生厅重点科研项目 (Z0 2 0 4)
