摘要
应用免疫组化法检测90例肝细胞癌与慢性旰病组织中 HBsAg 和癌胚抗原(CEA)。结果显示:(1)肝癌细胞中 CEA 的阳性率为55.0%,癌旁组织为22.5%;慢性肝病 CEA 检出率为10.2%,CEA 阳性反应与肝细胞癌关系密切,并与肝癌细胞分化有关。故检测 CEA 可为正确诊断肿瘤与估价病变发展提供佐证。(2)癌旁组织中 HBsAg 阳性率为85.0%,慢活肝组为81.8%,非慢活肝组为77.7%。HBsAg 阳性与肝病发生关系密切,提示两者似有因果关系。(3)在肝癌病变中CEA 阳性细胞存在于癌中,而 HBsAg 阳性细胞存在于癌旁组织中,两者在细胞学水平上虽无相关性,但都与肝癌的发生有关。可能为 HBsAg 损伤肝细胞,而使损伤的肝细胞修复,增生、癌变致 CEA 产生。
Primary Hepatocellular Carcinoma(PHCC)is usually accompanied by
Chronic Liver Disease(CLD)which is the important clue for the investiga-
tion of etiology and pathogenesis of PHCC.The appearance and distribution
of HBsAg and CEA in 40 cases of PHCC and its surrounding tissue and in
50 cases of CLD were detected by the Avidin-Biotin-Peroxidases Complex
(ABC)in specimens obtained from biopsies,autopsy,or operation,fixed
with formalin and embedded in paraffin.In PHCC group,CEA positive rea=
ction is higher than in its surrounding tissue and in the latter that is higher
than in the CLD group,55.5%,22.5% and 10.2%,respectively.This sug-
gests that CEA reaction of these tissues was closely related to carcinoma
developing and intensity of CEA reaction in PHCC was closely related to
tumor cell's differentiation.In the 50 cases of CLD and the 40 cases of
PHCC surrounding tissue,HBsAg positive reaction was 74.0% and 85.0%.Of
the PHCC group HBsAg positive was found in the surrounding tissue,CEA
positive was in the center of cancer.There are only 5 case(12.5%)of PHCC
with positive HBsAg in the cancer cells,and only 6 cases(10.2%)of
with CEA positive in the liver cells.It is proved that CEA and HBsAg are
related to the cancer developing.The CEA reaction can be used as a diagno-
stic and prognostic marker for PHCC.
出处
《南通医学院学报》
1992年第1期9-11,共3页
ACTA Academiae Medicinae Nantong
关键词
肝细胞癌
肝病
免疫组化
immunohistochemical method hepatocellular carcinoma chronic liver disease
CEA
HBsAg
