摘要
目的研究尖锐湿疣皮损中人类乳头瘤病毒 (HPV)基因含量及其临床意义。方法采用荧光定量PCR技术 (FQ PCR)检测尖锐湿疣皮损中HPV基因。分析皮损治疗次数与HPVDNA拷贝数的关系。结果尖锐湿疣发病高峰在年龄 4 0岁以下性活跃人群。 6 3例尖锐湿疣标本经FQ PCR检测后 5 0例(79.4 % )为HPV 6 / 11型阳性 ,其DNA模板平均拷贝数为 3.7× 10 4 ,HPV 6 / 11型阳性患者高频电平均治疗次数为 2 .17次 ,其拷贝数与治疗次数无明显相关性。结论HPV6 / 11型感染是尖锐湿疣发病的首要原因 ,HPV6 / 11型尖锐湿疣的预后与其皮损HPVDNA含量无明显相关性。
ObjectivesTo study the DNA contents of human papillomavirus (HPV) from the skin lesions of condyloma acuminata (CA) and its clinical significance.MethodsThe HPV DNA in skin lesions of CA was amplified by fluorescence quantitative polymerase chain reaction(FQ PCR), then the amplified HPV DNA was examined by close DA 620 type fluorescence analyzer. The relation between number of times in skin lesion treatment and copy number of HPV DNA was analyzed. ResultsSexually active young adults with age below 40 years old showed peak incidence of CA. Fifty out of sixty three CA specimens were confirmed to be HPV DNA 6/11 type positive (79.4%) by FQ PCR. The average copy numbers of DNA template in 6/11 type positive samples was 3.7×104 copies. The average number of times treated by electrofulguration was 2.17. There was no significant correlation of DNA copy numbers to times in treatment.ConclusionHPV infection, particularly 6/11 type HPV, is the cardinal cause of CA , there is no obvious correlation between its prognosis and HPV DNA contents of CA lesions.
出处
《医学临床研究》
CAS
2003年第8期569-571,共3页
Journal of Clinical Research
基金
湖南省科技厅资助项目 (编号 0 2ssy3 0 76)
