摘要
为探索糖尿病大血管并发症的发病机理,应用人生长激素对伴和不伴高脂血清培养的兔主动脉平滑肌细胞进行细胞动力学(以 DNA 增殖率为指标)和细胞形态学(光镜及透射电镜)检测。结果显示:人生长激素对平滑肌细胞非高脂血清组的增殖有明显抑制作用,且DNA 增殖率与生长激素浓度之间呈明显负相关(r=-0.49,P<0.01),相对应的,形态学上表现为平滑肌细胞不同程度蜕变。但高脂情况下,生长激素浓度为1～100ng/ml 时,则 DNA合成率明显上升,且细胞结构中细胞器增殖。在此时有特殊意义的是,平滑肌细胞间前胶原纤维大量出现。但当生长激素浓度达1000ng/ml 时,高脂血清组则呈细胞生长抑制作用。因此,本文结果提示:HGH1～100ng/ml 时,能促进动脉粥样硬化的形成。
With a view to unveil the mechanism of macrovascular disease (atherosclerosis)complicating diabetes mellitus,the effects of different doses of human growth hormone(HGH)on rabbit arterial smooth muscle cell (SMC)culture with or without hyperlipemic serum(HLS)were observed both cytodynamically(in terms of DNA replication)and morphologically under light microscope and transmission electron microscope.In the group without HLS,HGH suppresses the growth of SMC.The rate of disintegration of SMC correlates to the concentration of HGH added(r=-0.49,P<0.01). In the group with HLS,the effect of HGH varies with the dosage.HGH with a dose of 1-100 ng/ml stimulates the replication of DNA,with concomitant increase in subcellular structures and large quantities of pro-collagens appear between SMCs.At a dose of>1000 ng/ml,HGH suppresses cell growth.We conclude that atherosclerosis is favored by HGH at a dose of 1-100 ng/ml.
关键词
生长激素
平滑肌细胞
糖尿病
growth hormone
muscle cell
hyperlipemia
rabbits
