摘要
目的:建立一种简单的肝细胞分离、培养和鉴定方法。方法:取新生大鼠、成年小鼠的肝组织,立即剪碎、铜网过滤,RPMI-1640培养基培养,24小时后换液,除去血细胞后进一步培养,形态学观察,并用PAS(periodicac-id-Schiff)染色法鉴定。结果:在光镜可见分离、培养的肝细胞折光性强,呈圆形,偶见梭形,核大而圆,具有双核细胞;PAS染色后,胞质中布满粉红色糖原颗粒,核呈空泡状。结论:本实验方法可获得较纯的肝细胞,而且操作简便可行,经济实用。
Objective: To establish a simple method for the separation, culture and evaluation of hepatocytes. Methods: The liver tissue of new - born rats and mature mice was separated and cut into pieces immediately, filtered with copper grid and cultured with RPMI - 1640 culture medium. The blood cells were gotten rid of by changing fresh culture medium after 24 hours and the cells were cultured continually. The morphology of the cultured hepatocytes was observed and evaluated by using PAS (periodic acid Schiff) staining. Results: Under microscope, the separated and cultured hepatocytes reflect light well, usually round in shape. Nuclei are big and round, binucleate partly. There are full of pink glycogen particles in cytoplasm and vacuolar nucleus after PAS staing. Conclusion: The method can obtain purer hypatocytes and it is simple and economical for the experiments.
出处
《泸州医学院学报》
2003年第4期306-308,共3页
Journal of Luzhou Medical College
关键词
鼠
肝细胞
细胞培养
Mouse
Hypatocyte
Cell culture
