摘要
用电镜(EM)、聚丙烯酰胺凝胶电泳(PAGE)、酶联免疫吸附试验(ELISA)和协同凝集试验(CoA)等四种方法检测腹泻患儿粪便中轮状病毒,在20份四种方法全检标本中,EM 阳性13份,PAGE16份,ELISA14份,CoA16份。在70份PAGE、ELISA 和 CcA 三种方法检测标本中,PAGE 阳性率为76%,ELISA60%,CoA79%。以 PAGE 为参照,ELISA 和 CoA 两法的敏感性各为75%和91%,特异性各为88%和59%,诊断准确度各为79%和83%。本文对四种方法的优缺点作了评价。
A comparative study of electron microscopy(EM),polyacrylamide gel electrophoresis (PAGE),enzyme-linked immunosorbent assay(ELISA)and co-agglutination test (CoA)for detection of rotavirus(RV)in fecal specimens was carried out.Among 20 specimens tested by all the four methods,13 specimens were positive for RV by EM,16 by PAGE,14 by ELISA and 16 by CoA.Among 70 specimens tested by PAGE, ELISA and CoA,the positive rate was 76%,60%,and 79%,respectively.Compared with PAGE,the sensitivity of ELISA and CoA was 75% and 91%,the specitivity 88% and 59%,the diagnostic accuracy 79% and 83%,respectively.The advantages and disadvantages of the four methods are discussed,too.
出处
《南京铁道医学院学报》
1992年第4期249-251,255,共3页
Journal of Nanjing Railway Medical College
关键词
轮状病毒
微生物学检验
粪便
microsopy,electron
polyacrylamide gel electrophoresis
enzyme-linked immunosorbent assay
co-agglutination test
rotaviruses
