摘要
为了将生长抑索(Somatostatin,ss)基因融合到乙肝表面抗原(HBsAg 或 HBs)基因 C 末端 Acc Ⅰ 位点(aa223位置),先将质粒 pWR13-HBs/4的多接头区 Sal Ⅰ位点消除,因为它也可被 AccⅠ酶切。在AccⅠ位点加入 Bgl Ⅱ接头,用 Sac Ⅰ和 Bg Ⅱ切下 HBs 基因片段(700bp),并将其插入到 pUC12-ss/2中 ss 基因N 末端 Sac Ⅰ和 BamH Ⅰ位置.获得基因融合体质粒 pUC12-ss/HBs。经 DNA 序列分析,两基因融合处的阅读框架正确,且 HBs 基因的起始密码 ATG 和 ss 基因全序列都得以证实.
The hepatitis B surface antigen(HBsAg)particle has been successfully used as a carrier ofgenetic engineering vaccines for HIV,poliovirus,HSV-1,by means of the fusion of foreign gene withHBsAg gene.In this paper,the fusion of somatostatin(ss)gene with HBsAg gene was described. Firstly,a Bgl Ⅱ linker was added into the AccⅠsite corresponding to the aa 223 of HBsAg gene in pWR13-HBs/4.Then the HBsAg gene fragment(700 bp)obtained by digesting pWR13-HBs/4 with Sac Ⅰ and BglⅡ wasinserted into the Sac Ⅰ and BamHI sites right before the ss gene in pUCi2-ss/2.The reading frame,as wellas the initiation codon and terminator codon of the fusion gene in pUC12-ss/HBs was confirmed by DNAsequence analysis.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1992年第2期83-88,共6页
Journal of Nanjing Agricultural University
基金
农业部生物技术课题
