摘要
目的 :利用银杏外种皮分离制备银杏酸 ,并测定银杏酸含量。方法 :采用石油醚超声萃取和硅胶柱层析法从银杏外种皮中分离制备银杏酸 ;用高效液相色谱法测定银杏酸含量。色谱柱为HiQsilC18柱 ,流动相为甲醇 3%HAc溶液 (90∶10 ,V/V) ,流速 1ml/min ,检测波长为 310nm ,柱温 4 0℃。结果 :银杏酸在 1 14 4～ 5 72 0 μg范围内线性关系良好 (r=0 9978) ,平均回收率 97 5 0 % ,RSD为 1 70 %。结论 :可从银杏外种皮中分离制备银杏酸 ;银杏酸含量测定方法准确、可靠 ,可用于样品中银杏酸含量的分析。
Objective:To isolate ginkgolic acids (GA) from exopleura of Ginkgo biloba and to analyse GA. Methods:GA were ultrasonic extracted from exopleura of Ginkgo biloba with petroleum ether,and purified by silica gel column chromatography The content of GA in sample was determined by HPLC with methanol 3% acetic acid (90∶10) as mobile phase at a flow rate of 1 ml/min UV detection wavelength was set at 310 nm A HiQ sil C 18 column was used at 40℃ in analysis Results:A good linearity was obtained in the range of 1 144～5 720 μg(r=0 9978) for GA The average recovery of GA was 97 50% with RSD of 1 70% Conclusion:The method may be employed to prepare GA from exopleura of Ginkgo biloba The analysis method for GA is accurate and reliable,and can be used for determination of GA in sample
出处
《中药材》
CAS
CSCD
北大核心
2003年第8期557-559,共3页
Journal of Chinese Medicinal Materials
