摘要
目的探讨非协调性异种小动物模型中异基因胸腺修饰的可行性.方法供体三色豚鼠和受体SD大鼠各10只随机平均分成对照组(A组)和实验组(B组).A组大鼠接受3.5Gy全身照射,B组大鼠在此基础上3 d后接受5×106个豚鼠脾淋巴细胞胸腺内注射;两组大鼠分别在照射后18d接受豚鼠脾淋巴细胞腹腔内注射.结果接受胸腺注射后15d,B组混合淋巴细胞培养刺激效应低于A组(P＜0.05);3周后B组大鼠胸腺细胞表面仍有较高的豚鼠MHC-Ⅱ类抗原表达;腹腔注射后,B组中IgM类天然抗体水平的上升与A组相似,而IgG类上升明显受抑(P＜0.01).结论豚鼠-大鼠异种胸腺修饰可使受体新生T细胞对注入的抗原产生免疫抑制.
Objective To investigate the effect of T cells on the delayed xenograft rejection by in-
trathymic injection of xenogeneic antigen in the discordant xenotransplantation from Guinea-pig to rat.
Methods Guinea-pig and rat were, respectively, selected as donor and recipient. Denor (guinea-pig) and
recipient (SD rat) were divided randomly into two groups; the control group (group A) and the experi-
ment group (group B). All rats of two groups received whole body irradiation (3. 5 Gy) and were in-
traperitoneally injected with guinea-pig spleen cells (5×10~6) 18 days after irradiation for observing the
change of the natural antibody (IgM and IgG) in group B. Three days after irradiation, rats received in-
trathymic injection. Results The results of MLR showed that the stimulative effect in group A was
stronger than in group B (P < 0. 05) after 15 days of intrathymic injection. The expression of the MHC-
Ⅱ antigen of guinea-pig on rat's thymus cellular surface was (17. 02±5. 13)% after 3 weeks in group
B. After one week of intraperitoneal injection, in group A the level of natural antibody IgM and IgG was
ascended Obviously, but in group B, IgM elevated also, but IgG didn' t significantly raise (P < 0. 01).
Conclusion In the model of discordant xenotransplantaion from guinea-pig to rat, intrathymic inocula-
tion could induce immune suppression of T cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第8期742-744,共3页
Chinese Journal of Experimental Surgery
