摘要
目的 探讨抗独特型抗体佐剂疫苗对膀胱癌的免疫治疗作用。方法 用抗人膀胱癌抗独特型抗体佐剂疫苗 (Ab2 +SAF)免疫BALB/ C小鼠 ,即实验组 ;对照组用生理盐水 ,方法同实验组。免疫 3次后 ,于末次免疫后 1 w,将新鲜人膀胱移行细胞癌组织移植于小鼠肾包膜下 ,分别于移植后第 2、4、6、8和 1 0天处死小鼠 ,取血清进行抗抗独特型抗体 (Ab3)分析。移植瘤行组织学检查 ,观察宿主淋巴细胞浸润情况及瘤细胞可见率。结果 实验组小鼠肾脏包膜下人膀胱癌细胞系很快受到排斥 ,在移植后第 6天 ,淋巴细胞浸润即达高峰 ,而对照组在第 1 0天才达高峰 ;瘤细胞可见率 ,在移植后第 6天即明显降低 ,而对照组呈逐渐下降。实验组 Ab3为阳性 ,而对照组阴性。结论 Ab2 作为抗原免疫小鼠后 ,通过诱发或激活对人膀胱癌特异性体液和细胞免疫反应 ,排斥瘤细胞并抑制其生长。
Objective To study the role of anti idiotypic antibody in immunotherapy of bladder carcinoma and to investigate the effect of anti idiotypic antibody(Ab 2) on bladder carcinoma in vivo Methods BALB/C mice were immunized every week for 3 times with Ab 2 of bladder carcinoma which was connected with new type adjuvant SAF The control team were injected with salt water Fresh bladder tumor cells were transplanted to the subrenal capsule of immunized mice after the last injection Then the mice were bred separately on the 2nd,4th,6th,8th,and 10th days after the transplantation operations Anti Ab 2 (Ab 3) in sera was detected by using inhibition assay of enzyme linked immunospecific assay Tumor infilration lymphocytes and tumor cells from the tumor tissue of mice were observed microscopically Results The sera levels of Ab 3 in Ab 2 immunized mice were higher than those of the controls Tumor infilitration lymphocytes in the former were found to reach their highest peak on the 6th day,where as the highest peak for the controls was on the 8～10th days;tumor cell viability rate in the former decreased rapidly afrter 6 days,but in the controls decreased only gradually Conclusions Ab 2 could prevent growth of tumor after mice were immunized with Ab 2
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2003年第7期451-453,共3页
Chinese Journal of Gerontology
基金
卫生部科研基金资助 (98- 1 - 2 0 6)
