摘要
应用淋巴细胞单克隆抗体分离主要的淋巴细胞亚群,用间接免疫荧光法测定了癌病人外周血CD_4^+细胞的数量,显著少于正常人。分离了CD_4^+和CD_4^-细胞,分别用PHA激活及~3H-TdR掺入反映其功能变化,发现CD_4^+和CD_4^-细胞的掺入活性均比正常人显著降低。观察了正常人NK、CD_4^+、CD_8^+和B细胞对K_(562)肿瘤细胞的协同杀伤活性,以NK+CD_4^+细胞的活性最高。本文提示癌病人的CD_4^+细胞数量和功能下降,正常人CD_4^+细胞在协同杀伤肿瘤细胞中具有重要作用,说明CD_4^+细胞在肿瘤免疫中的重要意义。
Main subsets in lymphocytes ,NK、CD_4^+ 、CD_8^+ and B cells are isolated and investigated by using monoclonal antibodies. It is found that numbers of CD<4' cells in cancer patients are lower significantly than that in normal subjects. The CD_4^+ and CD_8^- cells in cancer patients are activited by PHA respectively. It shows the CPM of H-TdR incorporation in these two kinds of cells decreased obviously as compared with that of normal subjects. The synergistic killing activities of NK、CD_4^+、CD_8^+ and B cells on K_(562) tumor cells are determined. The killing activities of NK+CD_4^+ cells are more strong among them. The results show the CD_4^+ cells in cancer patients decrease both in quantity and quality.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第4期251-252,共2页
Immunological Journal
关键词
淋巴细胞亚群
肿瘤细胞
肿瘤免疫学
Lymphocytes subsets ,Monoclonal antibody ,Cancer patient ,Tumor cell
