摘要
利用IL-6分泌细胞株(人膀胱癌T_(24)细胞),建立了最佳天然IL-6产生条件和IL-6超诱生方法,IL-6分泌量最大可达15 576±1042.3μ/ml。同时对T_(24)细胞连续培养和冻存后IL-6产生水平进行了观察,表明该细胞分泌IL-6的能力十分稳定。
The optimal culture condition (initial concentration, passage interval, cryostorage, etc.) of human bladder cell line (T24) was studied, on which the spontaneous secretion of high value of interleukin-6 (IL-6) by T24 cells was observed. LPS, PMA, PJV and BCG promoted the IL-6 production by 9.03, 4.69, 2.00, 1.33 folds respectively. The 72.11 folds of IL-6 production was obtained by using superinduction method with PMA, cyclohexmide and actinomycin-D.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第2期102-103,共2页
Immunological Journal
基金
国家自然科学基金资助项目
编号3■00053
关键词
T24细胞系
IL-6
膀胱肿瘤
IL-6 production
T24 bladder cell line
Superinduction
