摘要
用SDS-聚丙烯酸胺凝胶电泳及Westen印迹方法检测已孕与未孕豚鼠抗GPCMV(豚鼠巨细胞病毒)蛋白血清抗体,观察了12只豚鼠初期感染期间,特定时间GPCMV蛋白抗体产生的动力学。实验结果表明,怀孕豚鼠抗体产生的时间比未孕豚鼠延迟并浓度较低,所有CMV感染的豚鼠血清中抗29.5KDa分子量病毒蛋白抗体最强,持续时间长,值得进一步研究。
Antibodies to guinea pig cytomegalovirus (CMV) proteins was examined in pregnant and non-pregnant guinea pigs by immunoblotting. Antibodies to 12 guinea pig CMV proteins appeared in a difined time course during primary infection and were detected at a later time and in lower concentration in pregnant than in non-pregnant guinea pigs.
In all animals examined, the protein with a migration rate of 29.5 KDa was most intense as indicated by the observation that all guinea pigs to this protein, reacted very strongly in immunoblots and retained this antibody.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1992年第1期28-31,共4页
Immunological Journal
关键词
巨细胞病毒
免疫印迹
初期感染
Cytomegalovirus, Primary infection, Immunoblotting, Anttibody
