摘要
采用高效液相色谱 -电喷雾电离串级质谱 (HPLC-ESI/MSn,n=1～ 2 )分别测量羽衣甘蓝及苔菜和样准样品中完型硫代葡萄糖的保留时间和负离子质谱 ,并用这种比较方法确认十字花科植物中 8种未知硫化葡萄糖甙。在 MS质谱中观察到硫代葡萄糖甙峰 [M-K]-,在 MS2 质谱中所有硫代葡萄糖甙都产生 m/z 75和m/z97的碎片峰 ,分别是 [S=C=N-OH]-和 [HSO4]-离子。另外还观察到了和硫代葡萄糖甙侧链有关的 [R-(C=NOH) -S]-离子。
The retention times and negative mass spectra of intact glacosinolates extracted from the kale and tai tsai and the reference samples are measured by high peformance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI/MS n,n=1～2), respectively. The unknown chromatography peaks in eight species of glucosinolates were certified by the sqnare peaks to normalization. Negative quasimolecular ion mass spectra were observed. Daughter ions at m/z 75 and m/z 97 present in the spectra of all the GS investigated and its are - and - moieties, respectively. Ions [R-(C=NOH)-S] -, which provides side chain information, were also observed.
出处
《质谱学报》
EI
CAS
CSCD
2003年第3期385-388,共4页
Journal of Chinese Mass Spectrometry Society
基金
北京市自然科学基金 (6 0 0 2 0 0 6 )
北京市新星科技项目 (95 5 810 0 4 0 0 )
