摘要
目的:探索取材于乳腺癌患者外周血诊断微转移的方法。方法:66例乳腺癌和37例乳腺良性疾病患者的外周血,分离其有核细胞后进行细胞总RNA的抽提,运用RT-PCR技术进行CK-19 mRNA的检测。结果:以RT-PCR终产物出现460bp条带定为阳性。66例乳腺癌患者CK-19mRNA有24例表达,总阳性率为36.36％。37例乳腺良性疾病者CK-19mRNA无一例表达。两组有统计学意义(x2=17.54,P<0.001)。结论:RT-PCR检测CK-19 mRNA可以用于临床诊断乳腺癌患者外周血中微转移。
Purpose: Detect cytokeratin 19 mRNA(CK-19 mRNA) in nucleated cells in peripheral blood from breast cancer. To establish a diagnostic method for breast cancer metastasis in peripheral blood. Methods: Peripheral blood samples in breast cancer patients (test group, n = 66) and benign tumour in breast patients ( control group, n = 37) were taken. Then, the nucleated cells were separated and total RNA extracted, and CK-19 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) technique. Results: Samples were diagnosed CK-19 mRNA positive when 460 bp band appeared in RT-PCR end-product. The positive rate of CK-19 mRNA is 36. 36 % (24/66) in test group . None of the benign tumour breast patients expressed CK-19 mRNA. The difference between the two groups was statistically significant (P <0.001). Conclusions: RT-PCR can be used to diagnose breast cancer micro metastasis.
出处
《中国癌症杂志》
CAS
CSCD
2003年第4期301-303,共3页
China Oncology
基金
甘肃省科学事业费项目(编号:QS992-C33-023-14)
