摘要
目的:检测趋化因子IL-8、GM-CSF、RANTES、MCP-1在乳腺癌患者及正常志愿者外周血中的浓度并探讨其临床意义。方法:酶联免疫吸附试验法(ELISA)检测93例乳腺癌患者及20例正常人外周血中趋化因子的浓度,以及临床病理相关性分析。结果:乳腺癌患者外周血中IL-8、RANTES和MCP-1的浓度显著高于正常对照组,且在临床Ⅲ期患者较Ⅰ期患者显著升高,IL-8、GM-CSF、RANTES和MCP-1预测乳腺癌远处转移的敏感性分别为88.6％、28.6％、71.4％、60.0％,特异性分别为82.8％、93.1％、60.3％、82.8%,,IL-8、GM-CSF、RANTES和MCP-1的表达具有相关性。IL-8、MCP-1阳性组的乳腺癌患者生存率低、预后差。结论:监测乳腺癌患者外周血中趋化因子IL-8、MCP-1、RANTES的浓度可作为预测乳腺癌转移的较理想指标,IL-8、MCP-1高表达者预后较差。
Purpose: To investigate the plasma levels of chemokine IL-8, GM-CSF, RANTES and MCP-1 in patients with breast carcinoma and in healthy controls and to explore its clinical, pathologic and diagnostic significance. Methods: Enzyme linked immunosorbent assay ( ELISA) kits were used in 93 breast cancer patients and 20 health controls, and clinical significance was analysed. Results: The plasma levels of IL-8, RANTES and MCP-1 were significantly higher in the patients than in<the healthy controls, and plasma levels of IL-8, MCP-1 and RANTES were significantly higher in patients with stage HI disease than those in patients with stage I. For the predictive value of distant metastasis, the sensitivities of IL-8, GM-CSF, RANTES, MCP-1 were 88.6%, 28.6%, 71. 4% and 60.0% respectively, and the corresponding specificities were 82. 8%, 93. 1%, 60. 3% and 82. 8%. Significant correlations were found between IL-8, GM-CSF, RANTES and MCP-1. Overall survival of breast cacer patients subdivided by positive IL-8 and MCP-1 group were lower than the negative group. Conclusions: This study indicates that the increased plasma levels of IL-8, MCP-1, and RANTES, might be valuable predictive markers for identifying metastatic breast cancer patients, and high expression of IL-8, MCP-1 as predictive markers of poor prognosis also.
出处
《中国癌症杂志》
CAS
CSCD
2003年第4期297-300,共4页
China Oncology
基金
国家杰出青年科学基金(30025015)
上海市卫生系统百人计划资助项目(98BR015)
