摘要
单细胞分析对重大疾病的早期诊断、治疗和药物筛选以及细胞生理、病理过程的研究有重要意义[1].将毛细管电泳用于单细胞多组分的测定已取得一些成果, 但受毛细管的一维结构限制, 单细胞进样和溶膜操作较复杂[2,3].
A microfluidic system was developed for the analysis of single biological cells, with functional integration of cell sampling, single cell loading, docking, lysing, and capillary electrophoretic separation in microfabricated channels on a single glass chip. Channels were 12 μm deep and 75μm wide, with a double-T design cell injector that was directly connected to a capillary electrophoretic separation channel with an effective separation length of 37 mm. During sampling with a cell suspension (cell population 1. 2×105 cells/mL in physiological salt solution), differential hydrostatic pressure (created by adjusting liquid levels in the four reservoirs) was used to control cell flow exclusively through the 200 μm channel between the two T-junctions. Single cell loading into the separation channel was achieved by electrophoretic means by applying a set of potentials at the four reservoirs, counteracting the hydrostatic flow. A special docking (adhering) procedure for the loaded cell was applied through flow control to affect cell lysis at the applied CE separation voltage(1. 4 kV) within the working electrolyte(pH 9. 2 borate buffer) without additional lysates. The docked lysing approach reduced dispersion of released intracellular constituents, and significantly improved the CE separation efficiency. FITC-labeled components in the cellular membrane of single human erythrocyte cells were detected by using laser induced fluorescence. A retention time precision of 0. 9% RSD(n = 4) for FITC, and an average separation efficiency of 18 μm plate height for FITC were achieved.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第9期1582-1584,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20299030)
