摘要
构建口蹄疫融合表位基因 - SG表达载体 p ET- SG,转化重组子与 K80 2 ,新鲜过夜菌以 2 %接种量接种 2 YT培养液 ,37℃培养 2 h后用 IPTG诱导 ,每隔 1h取样 ,共 8h。根据 SDS- PAGE结果 ,口蹄疫融合表位基因 - SG在 p ET原核表达系统可获得较高表达 ,用 IPTG诱导 4 h蛋白表达量最高 ,用 Ni+柱亲和层析可得到较纯蛋白。
Proeukaryotic expression vector pET SG for fusion epitopes of foot and mouth disease virus(FMDV) was constructed and transferred into K802.Fresh bacterium was added to 2YT culture medium at a radio of 2% and the system was incubated for 2 h and then induced by IPTG.Expression products was collected every hour and the best expression time was decided by SDS PAGE.FMDV epitope genes SG was expressed with high yield in pET proeukaryotic system,K802 pET SG produced the largest amout of fusion protein when induced by IPTG for 4 h, and pure fusion proteins was obtained through a Ni + affinity chromatography column.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第8期904-905,共2页
Academic Journal of Second Military Medical University
基金
国家"8 63"计划资助项目 ( 2 0 0 1AA2 13 111)
