摘要
目的 :测试海洋生物活性物质总草苔虫内酯对严重急性呼吸综合征相关冠状病毒 (SARS- Co V )的作用。 方法 :以Vero- E6细胞作为病毒宿主细胞 ,以细胞致病效应 (CPE)和感染细胞保护率 (CPR)为检测指标 ,测试海洋来源的化合物总草苔虫内酯对 SARS- Co V感染细胞的保护作用。 结果 :在 4、2 0、10 0μg/ m l 3个质量浓度下 ,总草苔虫内酯预防 SARS- Co V感染的 CPE分别是 、 、 ,CPR分别是 7%、6 %、39% ;总草苔虫内酯治疗 SARS- Co V感染的 CPE分别是 、 、 ,CPR分别是 33%、5 8%、4 0 %。 3个质量浓度总草苔虫内酯的细胞毒性率均不超过 5 0 %。 结论 :在体外筛选模型中 ,总草苔虫内酯在4μg/ m l以上浓度时显示有一定的对抗 SARS- Co
Objective: To observe the anti SARS virus effect of total bryostatins, a mixture of 9 bryostatins isolated from marine animal Bugula neritina . Methods: Vero E6 cells were used as SARS virus host cells. Cytopathic effect (CPE) and cell protection rate (CPR) were used to determine the protective effects of total bryostatins against SARS virus. Bryostatins at concentration of 4, 20 and 100 μg/ml were tested separately in 2 experiments.Results: In the provention test, CPE were ,,; CPR was 7%, 6%,39%; in the treatment test, CPE were ,,; CPR were 33%, 58%, 40%. Conclusion: The results indicate that bryostatins with a concentration over 4 μg/ml has anti SARS activity and protection action of SARS infected cell.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第8期821-822,共2页
Academic Journal of Second Military Medical University
基金
上海市科委新药重点基金 ( 0 14 3 193 2 0 )
