摘要
目的观察原代培养神经元在红藻氨酸(KA)作用下细胞表面形态的三维构像变化。方法利用原子力显微镜(AFM)对大鼠海马神经元经不同浓度的KA(25和250 μmol/L,50和100 nmol/L)分别作用10 min和100 min后的表面结构进行纳米级水平扫描和观测。结果正常海马神经元表面光滑,起伏均匀、规律;KA作用后神经元呈退行性改变, 表现为胞体肿胀,胞膜表面粗糙,出现隆起和“孔洞”样结构,并且其变化程度分别与作用时间和KA浓度呈量-效关系。结论KA对海马神经元毒性作用后胞膜表面超微结构所产生的明显变化,可借助AFM清晰观察,并定量分析。
Objective To observe the three-dimensional morphological changes on the membrane surface of primary cultured rat hippocampal neurons in response to kainic acid (KA) exposure. Methods After isolation and primary culture, Wistar rat hippocampal neurons were treated with KA at the concentrations of 0, 25, and 250 μmol/L for different durations (10 and 100 min) to observe the subsequent changes in the membrane surface structure of the neurons by nano-scale scanning with an atomic force microscope (AFM). Results Normal neurons displayed smooth membrane surface with even and regular undulation, while the neurons treated with KA, in contrast, presented degenerative changes characterized by cell swelling and coarse mem-brane surface with processes and holes. As the treatment was prolonged and KA concentration increased, the changes became more evident. Conclusion As a result of the toxic effect of KA, the membrane surface ultrastructure of rat hippocampal neu-rons undergo obvious changes, which can be clearly observed and quantitatively analyzed by means of AFM.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第7期659-662,共4页
Journal of First Military Medical University
基金
军队十五重大临床技术项目(2002卫医字18号-15)~~
关键词
红藻氨酸
海马神经元
胞膜表面
超微结构
原子力显微镜观察
atomic force microscope
cytomembrane /ultrastructure
hippocampus/neuron
kainic acid/drug effects
