摘要
应用单克隆抗体免疫荧光技术(MIF)检测了青枯病在马铃薯种薯上的潜伏侵染。测试结果表明,MIF检测青枯病茵的灵敏度为2×10~3～2×10~4细菌/毫升。787.5倍荧光显微镜,每视野平坶有5个以上典型荧光细菌,即可判别为带菌(阳性),而1～4个荧光细菌则视为可疑,需作生物测定。从种薯脐部取样,经组织匀浆后采用低、高速离心收集细菌体,可提高样品青枯菌的检出率。
Theroutine method(Oil content:'remains method'; Protein content: Kajadal method)and the near-IR method for determination for coarse oil and protein contents were compared and analysed in this paper. The results indicated that: the coarse oil and protein contents ofsoybeans determined by routine method and that by the near-IR was notsignificantly different. Therefore, the near-IR could be substitute for the routine method, The colour of soybean seed coat had no effect on accuracy of determination by the near-IR.
出处
《马铃薯杂志》
1992年第1期2-6,共5页
Chinese Potato Journal
