摘要
利用F2 代设计建立了由 2 16头F2 代个体组成的长白×蓝塘猪资源群 ,应用PCR RFLP方法检测F2 代个体IGF 1基因的多态性 ;通过固定模型方法估计了IGF 1不同基因型的最小二乘均值 ,同时采用混合模型方法分析不同性状IGF 1基因型的遗传效应 ,并由此计算IGF 1基因的加性和显性效应。固定模型和混合模型方法的分析结果都表明 ,IGF 1不同基因型对猪断奶后日增重有显著影响 ,AA型的遗传效应显著比AB型高 2 0 5 8g(P =0 0 347) ,IGF 1基因对该性状的加性和显性效应分别为 1 78g和 - 18 81g ;对胴体组成性状也有影响 ,AA型和AB型对骨率的遗传效应分别极显著低于BB型 5 2 2 % (P =0 0 0 0 8)和 5 19% (P =0 0 0 0 7) ,IGF 1基因对该性状的加性和显性效应分别为 - 2 6 1%和 - 2 5 8% ;AA型胴体瘦肉量的遗传效应显著比AB型少 0 4 5kg(P =0 0 2 6 4 ) ,IGF 1基因对该性状的加性和显效应分别为 0 16kg和 0 6 1kg ;AA型和AB型皮脂率的遗传效应比BB型分别高 8 81%(P =0 0 2 0 6 )和 7 6 4 % (P =0 0 4 31) ,对该性状的加性和显性效应分别为 4 4 1%和 3 2 4 %。因此 ,IGF 1基因的遗传效应分析说明该基因对断奶后日增重、骨率、胴体瘦肉量和皮脂率等 4个性状有显著的遗传影响 ;而加性和显性效应估计值的大?
A Landrace × Lantang resource population (LL SCAU) including 216 F 2 pigs was founded by F 2 design for analysis of IGF 1 gene polymorphism by PCR RFLP.The least square means of divergent IGF 1 genotypes for the measured traits were estimated with the fixed model.The genetic effects of IGF 1 gene were estimated with a mixed model and the additive and dominant effects of IGF 1 gene were accordingly calculated.The results of the fixed model and the mixed model showed that IGF 1 locus significantly affected average daily gain after weaning.Average daily gain after weaning for AA genotype on IGF 1 locus was 20 58 g ( P =0 0347) higher than for AB .The additive and dominant effects attributed to IGF 1 were 1 78 g and -18 81 g respectively. IGF 1 locus also significantly affected carcass composition.Bone percentages for AA and AB on IGF 1 locus were lower than for BB by 5 22% ( P =0 0008) and 5 19% ( P =0 0007) respectively,and the additive and dominant effects were -2 61% and -2 58% respectively.Amount of carcass lean for AA on IGF 1 locus was less than for AB by 0 45 kg ( P =0 0264),and the additive and dominant effects were 0 16 kg and 0 61 kg respectively.Skin and fat percentage for AA and AB on IGF 1 locus was higher than for BB by 8 81% ( P =0 0206) and 7 64% ( P =0 0431) respectively,and the additive and dominant effects were 4 41% and 3 24% respectively.The genetic analysis of IGF 1 gene showed that IGF 1 locus significantly affected average daily gain after weaning,bone percentage,carcass lean,skin and fat percentage.The estimated additive and dominant effects showed that only the additive effect of IGF 1 locus on skin and fat percentage was much higher than dominant effect,and IGF 1 gene can be used as a major gene for effective selection of these traits.
基金
国家重点基础研究规划项目 (编号 :G2 0 0 0 0 1610 4)
国家自然科学基金 (编号 :3 980 0 10 3 )
广东省自然科学基金 (编号 :980 15 3 )资助~~
