摘要
以林檎叶的水提取组(A)、乙醇提取经石油醚萃取后分成的石油醚组(B)和乙醇提取组(C)、C组加HCl溶解后得到的(D)组、酸不溶物经乙酸乙酯溶解经NaOH萃取得NaOH萃取组(E)、乙酸乙酯萃取组(F)及石油醚提取组(G)为受试物,用小鼠免疫器官重量法、小鼠常压耐缺氧实验、小鼠游泳实验、小鼠耐高温、耐低温实验和小鼠碳粒廓清法实验,探讨了林檎叶不同成分对机体免疫功能的影响.结果表明:A组比对照组小鼠胸腺指数和脾脏指数均有显著增加(P<0.05),C组比对照组胸腺指数有显著的增加(P<0.05);C组浓度增加对胸腺指数有极显著的影响(P<0.01),脾脏指数与对照相比有显著差异(P<0.05);A组廓清指数、吞噬活性均比对照有明显提高(P<0.05);A、C、D与对照组比较能显著延长小鼠耐缺氧时间(P<0.05);C、D、E组比对照组延长小鼠耐低温时间(P<0.05);D组比对照能延长小鼠游泳时间(P<0.01);受试物均无明显提高小鼠的抗高温能力.林檎叶的水提取物和乙醇提取物能明显提高小鼠的免疫功能.
The extracts of Malus doumeria leaves were separated into 7 groups, i.e. waterextracted (A), petroleum etherextracted (B), ethanolextracted (C) divided on ethanol extracts by petroleum ether, HClextracted (D) and insoluble part in HCl divided on the group C by HCl, NaOHeatracted (E), ethyl acetateextracted (F) divided on the insoluble part in HCl by NaOH, and ethyl acetateextracted (G). The methods of weight of mice's immune organs, carbon clearance, enduring meager oxygen and enduring low and high temperature were used. The effects of Malus doumeri leaf extracts on mice's immune function were investigated. The research results indicate that compared with the control, group A can significantly increase the thymic and splenic index of mice (P<0.05), group C can significantly increase the thymic index of mice (P<0.05), and the concentration of group C has significant effect on the thymic index (P<0.01) and splenic index (P<0.05), that group A can significantly increase swallow index and swallow coefficient (P<0.05), groups A、C and D can significantly prolong the time of enduring meagre oxygen (P<0.05), groups C、D and E can significantly prolong the time of enduring low temperature(P<0.05) and group D can significantly prolong the time of swimming (P<0.01), and that all the groups have obviously no effect on the capability of enduring high temperature. It is concluded that Malus doumeri leaf extracts can improve the immune function of mice.
出处
《中南林学院学报》
CSCD
2003年第4期28-31,共4页
Journal of Central South Forestry University
基金
湖南省自然科学基金项目"林檎保健成分及其机理研究"(编号为98JJY2012)
