摘要
目的 :探讨银杏叶提取物 (Egb761 )对豚鼠哮喘模型血红素氧合酶 1 (HO 1 )表达的影响。 方法 :将 30只豚鼠随机分为 3组 (n =1 0 ) :(1 )正常组 ;(2 )哮喘组 ;(3)治疗组。测定全血一氧化碳血红蛋白 (COHb)的百分比含量、气道阻力并观察气道壁嗜酸性粒细胞 (EOS)浸润情况 ,用免疫组织化学染色方法观察HO 1在豚鼠肺组织中的表达变化。结果 :各组气道上皮细胞HO 1阳性表达的平均吸光度分别为 0 .1 70± 0 .0 2 0、0 .70 7± 0 .0 58、0 .397± 0 .0 34。哮喘组HO 1的表达水平显著高于正常组 (P <0 .0 1 )。治疗组HO 1的表达水平显著低于哮喘组 (P <0 .0 1 )。结论 :银杏叶提取物能显著抑制哮喘豚鼠气道壁内上皮细胞HO 1的表达 ,提示银杏叶提取物抑制HO
Aim: To explore the effect of the ginkgo bioba extract on the expression of heme oxygenase 1(HO 1) in bronchial asthma. Methods: 30 guinea pigs were randomly divided into 3 groups( n =10): ①Normal control group; ②Asthmatic group; ③Therapeutic group. Blood carbon monoxide Hb(COHb) percent value, Airway resistance and eosinophilic inflammation of airway wall were observed, the expression of HO 1 in lung tissue were observed by immunohistochemical staining. Results: The expression of HO 1 was mainly located in airway epithelium in these 3 groups, the optical densities were 0.170±0.020?0.707±0.058?0.397±0.034, respectively. The asthmatic group showed higher optical densities than that of the normal control group( P <0.01), and the therapeutic group showed lower optical density than asthmatic group( P <0.01).Conclusion: The expression of HO 1 is inhibited significantly by the treatment of the ginkgo bioba extract, which may be one of the mechanism for treating asthma by ginkgo bioba extract.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2003年第3期302-305,T002,共5页
Chinese Journal of Applied Physiology
基金
广西省科技厅资助项目 (桂科攻 0 14 30 6 0 )
广西省卫生厅资助课题 (Z2 0 0 0 14 )
