摘要
目的和方法 :应用血管内皮剥脱后再狭窄模型 ,动态观察胸腹主动脉壁核膜核苷三磷酸酶及核酸代谢和糖代谢相关酶 5’ 核苷酸酶、腺苷脱氨酶和琥珀酸脱氢酶活性的变化。探讨其与血管平滑肌细胞增生和新生内膜形成的关系。结果 :血管内皮剥脱后 ,胸腹主动脉壁核膜核苷三磷酸酶活性持续升高 ,与血管内膜增厚程度相平行 ;血管平滑肌细胞收缩型标志蛋白α 肌动蛋白表达降低及合成型标志蛋白骨桥蛋白表达上调 ,说明血管平滑肌细胞发生了表型转化 ,由分化型转变成为去分化型 ;5’核苷酸酶、腺苷脱氨酶和琥珀酸脱氢酶活性表现为先升后降 ,三种酶活性均于血管平滑肌细胞增殖旺盛期 (术后 3～ 7d)达峰值。结论 :细胞内参与mRNA转运及糖。
Aim and Methods: To determine the relationship between the nuclear envelope nucleoside triphosphatase (EC 3.6.1.15, NTPase) activity and the phenotypic modulation of vascular smooth muscle cell (VSMC), the NTPase activity was detected during restenosis after de endothelialization in vascular wall. The activities of three enzymes involved in carbohydrate and nucleic acid metabolism were also investigated by spectrophotometry. Results: The activity of NTPase increased continuously and associated with the process of intimal thickening. Western blotting showed that expression of SMα actin, as the marker of contractile phenotype of VSMC, decreased continuously. Osteopontin (OPN), the marker of synthetic phenotype of VSMC, was up regulated during the process. These suggested that intimal injury induced phenotypic modulation of VSMC. The activities of 5' nucleotidase, adenosine deaminase and succinate dehydrogenase increased and reached their peaks on 7 days after de endothelialization. The changes of three enzymes were associated with proliferation in VSMC. Conclusion: The efflux of mRNA and the changes of enzyme activity involved in carbohydrate or nucleic acid metabolism may be the biochemical basis in the development and progression of restenosis.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2003年第3期241-244,T001,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金资助项目 (30 270 4 99)
河北省自然科学基金资助项目 ( 30 1358)
