摘要
以伪狂犬病病毒上海株 (PRV SH)细胞感染物为模板 ,PCR扩增出 1 2 3kb的EP0基因完整编码区片段 ,将该基因片段克隆到pGEM T easy中 ,经过双脱氧末端终止法序列测定 ,并同国内的PRV Ea株进行同源比较 ,发现PRV SH株EP0基因存在多处突变和一处插入。进一步将该片段插入到原核表达载体 pET32a (+)的His Tag下游 ,构建了的原核表达质粒 pETEP0 ,为今后深入研究该基因的表达及其功能奠定了基础。
The EP0 ORF of pseudorabies virus Shanghai strain was obtained by polymerase chain reaction (PCR) using the cells infected with PRV-SH as template, subsequently cloned into pGEM-T-easy vector. The nucleotide was compared with the corresponding sequence of PRV-Ea strain. There were several mutant and a insertion in EP0 gene of PRV-SH strain. Then, the EP0 gene was cloned into pET32a(+), downstream of His-Tag, and the pETEP0 expression vector was constructed, which established a base for further study of expressing and function of the EP0 gene.
出处
《畜牧与兽医》
北大核心
2003年第8期8-10,共3页
Animal Husbandry & Veterinary Medicine
