摘要
目的 :探讨P物质 (substanceP ,SP)作用于离体培养的大鼠肉芽组织成纤维细胞后 ,对成纤维细胞转化生长因子 β- 1(TGFβ - 1)及其受体 - 1/ - 2 (TGFR - 1/R - 2 )基因表达的影响。方法 :采用大鼠肉芽组织成纤维细胞培养和逆转录 -聚合酶链反应 (RT -PCR)技术 ,观察SP在不同浓度 (10 -9M～ 10 -5M )及不同孵育时间 (0h ,3h ,6h ,12h ,2 4h)情况下刺激成纤维细胞后 ,成纤维细胞TGFβ - 1/R - 1/R - 2mRNA表达的改变情况。结果 :SP可上调大鼠肉芽组织成纤维细胞TGFβ - 1/R - 1/R - 2mRNA的表达。其剂量 -效应曲线呈双相分布 ,最大效应浓度为 10 -8M。在最大效应浓度 (10 -8M )时 ,SP对大鼠成纤维细胞TGFβ - 1/R - 1/R -2mRNA表达的上调作用分别于刺激细胞后 6h/ 6h/ 12h达高峰。结论 :体外实验结果显示 :SP对大鼠肉芽组织成纤维细胞TGFβ - 1/R - 1/R - 2基因表达存在直接影响 ,其表现形式与SP的刺激浓度及孵育时间有关。这种影响可能是创伤愈合过程中SP影响成纤维细胞增生、分化和瘢痕组织形成的机制之一。
Objective:To probe into the effect of substance P (SP) on transforming growth factor β - 1 (TGFβ - 1 ), transforming growth factor receptor- 1 (TGFR- 1 ) and transforming growth factor receptor-2 (TGFR-2) gene expressions in cultured rat granulation tissue fibroblasts in vitro. Methods:Semi-quantitative RT-PCR was used to observe changes of TGF β-1, TGFR-1 and TGFR-2 gene expressions in cultured rat granulation tissue fibroblasts treated with SP under different stimulating concentrations (10 -9 M～10 -5 M) and incubating time points (0h, 3h, 6h, 12h, 24h).Results:SP could up-regulate TGFβ -1, TGFR-1 and TGFR-2 gene expressions in rat granulation tissue fibroblasts in vitro. Their response curves displayed a two-way distribution which have a most effective dot in 10 -8 M and the effect that SP imposed on TGF β-1/ R-1/ R-2 gene expressions achieved peak points after treated rat fibroblasts with 10 -8 M SP for 6h, 6h, 12h respectively.Conclusions:SP has a direct effect on TGFβ-1, TGFR-1 and TGFR-2 gene expressions in rat granulation tissue fibroblasts in vitro and it has a relationship with different stimulating concentrations and incubating times of SP. This effect maybe one of the mechanisms about fibroblast proliferation, differentiation and scar tissue formation regulated by SP during wound healing.
出处
《中国现代医学杂志》
CAS
CSCD
2003年第12期5-8,共4页
China Journal of Modern Medicine
基金
国家重点基础研究发展规划专项经费资助项目 (G19990 5 42 0 4)
