摘要
目的 评价抗核小体抗体 (AnuA)对系统性红斑狼疮 (SLE)诊断的敏感性和特异性 ,并了解其与SLE活动性的关系及与其他自身抗体的关系。方法 用酶联免疫吸附 (ELISA)测定方法检测SLE患者、疾病对照组 (包括原发性干燥综合征、多发性肌炎、皮肌炎、系统性硬化症 )和正常对照组血清中的AnuA ,并记录SLE患者的各种临床表现及实验室指标 ,分析其与AnuA的关系。结果 10 3例SLE患者中 6 9 9%血清AnuA阳性 ,6 6例疾病对照组仅 3 0 %阳性 ,30名正常对照组全部阴性 ;SLE组患者AnuA阳性率显著高于疾病对照组和正常对照组 (P <0 0 1) ,AnuA在SLE中检测的敏感性和特异性分别为 6 9 9%和 97 9%。AnuA阳性组的SLE患者肾损害、皮肤损害的发生率(6 1 1%、70 8% )明显高于AnuA阴性组 (2 9 0 %、32 3% ) (P <0 0 5 ) ;AnuA阳性组与AnuA阴性组相比 ,在年龄、性别、病程上差异无显著性 (P >0 0 5 )。AnuA滴度的高低与SLE患者的SLEDAI评分有明显相关性 (r=0 2 82 ,P <0 0 5 )。抗dsDNA抗体、抗Sm抗体、快速狼疮因子 (DNP)、抗组蛋白抗体 (AHA)阴性的SLE患者AnuA的阳性率分别为 6 5 6 %、6 8 0 %、6 3 9%、6 4 1%。结论 AnuA对SLE诊断的敏感性高、特异性强 ;它与SLE疾病活动性密切相关 ,对抗dsDNA抗体、Sm。
Objective To evaluate the diagnostic specificiy and sensitivity of antinucleosome antibodies (AnuA) on systemic lupus erythematosus (SLE),and to analyze the relationship between AnuA and activity of SLE.Methods The enzyme linked immunosorbent assay (ELISA) was used to measure the AnuA in serum of 103 SLE patients,66 patients with other rheumatic diseases (including primary Sjgren syndrome,polymyositis,dermatomyositis and systemic sclerosis) and 30 healthy controls.Results Of the 103 SLE patients,69 9% were seropositive for AnuA.Only 3 0% of 66 control patients were seropositive ( P <0 01);The sensitivity and specificity of AnuA in SLE were 69 9% and 97 9%,respectively.The incidences of renal disorders and skin lesions in AnuA positive SLE were 61 1% and 70 8% respectively,and significantly higher than those of AnuA negative SLE (29 0% and 32 3%, P <0 05);The titer of AnuA was associated with SLEDAI ( r =0 282, P <0 05);There was no significant difference in age,sex and disease duration between positive and negative AnuA of SLE ( P >0 05).The incidences of AnuA in SLE with negative anti dsDNA,Sm,anti deoxyribonucleoprotein antibody (DNP) and anti histone antibody (AHA) were 65 6%,68 0%,63 9% and 64 1%,respectively.Conclusion AnuA is one of the most valuable marker in diagnosis of SLE;AnuA has a close relationship with the activity of SLE;AnuA is valuable in diagnosis of SLE with negative anti dsDNA,Sm,DNP and AHA antibodies.
出处
《中华风湿病学杂志》
CAS
CSCD
2003年第8期474-477,共4页
Chinese Journal of Rheumatology
