摘要
目的 探讨鸡卵黄特异抗体 (IgY)对健康人牙菌斑中变形链球菌构成比的影响。方法 选择志愿受试者 2 4人 ,随机分为 3组 :实验组 (9人 )使用 1 5 %IgY ;阳性对照组 (9人 )使用 1 7mmol L洗必太溶液 ;阴性对照组(6人 )使用PBS缓冲生理盐水 ,各组均于饭后用药含漱 1min ,持续 3周。分别于用药前、用药中 1、2、3周及停药后1、3、5周记录菌斑指数 ,并收集光滑面集合菌斑样本在MS及MSB培养基中微需氧环境培养 ,检测菌斑中口腔链球菌群及变形链球菌的菌落形成数 ,分析各组使用药物前后菌斑中变形链球菌所占比例的变化及变化的延续性。结果 应用IgY后 ,其菌斑指数无明显改变 ,但牙菌斑中变形链球菌群在口腔链球菌群中的比例明显降低 ,并且在较长时间内 (停药后 5周 )保持菌斑中变形链球菌的低水平。结论 IgY处理后牙面菌斑的菌群微生态发生改变 ,为进一步研究牙菌斑微生态防治打下基础。
Objective To evaluate the inhibitory effects of topical application of specific immunoglobulin yolk antibodies (IgY) to mutans streptococci on composition of human dental plaque.Methods A total of 24 healthy adult volunteers were selected and randomly distributed into three experimental groups. After a supragingival scaling, the subjects were treated with an application of IgY containing spray (test group,n=9), a 0.15% chlorhexidine(CH) spray(positive control group,n=9) or a placebo phosphate buffered saline(PBS) spray without any active ingredients(negative control group,n=6) three times daily for three weeks. Pooled plaque samples from the smooth surfaces of teeth were collected for microbiologic analysis before administration (baseline,day 0)、during treatment(days 7、14 and 21)and after withdrawal of the agents(days 28、42 and 56)and plaque index were recorded.Microbial cultivation were performed by serially diluting the samples with PBS solution and cultivating the aliquots on mitis salivarius agar(MS) and MS supplemented with bacitracin (MSB) for oral streptococci and mutans streptococci respectively. The number of colony-forming unit (CFU) per plate was counted and the percentage of mutans streptococci per oral streptococci in dental plaque were determined. The effect of IgY on colonization of mutans streptococci were analyzed by an analysis of variance(ANOVA).Results The plaque indexes remained unchanged,but mutans streptococci in dental plaque was suppressed significantly following a short term(three weeks) application of IgY, and low level of mutans streptococci persisted for at least 5 weeks after withdrawal of IgY.Conclusion The results suggest that the immunization with specific immunoglobulin yolk against mutans streptococci could be useful for long term suppression of mutans streptococci colonization in human dental plaque.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第4期295-297,共3页
West China Journal of Stomatology
关键词
鸡卵黄特异抗体
人牙
菌斑
变形链球菌
影响
体内研究
immunoglobulin yolk antibodies(IgY)
dental plaque
mutans streptococci
oral streptococci
